Biotin, also known as vitamin H is an important nutritional factor and animal feedadditive. Biotin occurs naturally in milk, dairy products, eggs, oat flakes and nuts, to state but a few. It is highly important for the metabolism of humans and animals, as it is involved as a coenzyme in important processes such as gluconeogenisis, biosynthesis of fatty acids and the metabolism of amino acids. Biotin deficiency causes inhibition of growth in mammals, loss of appetite and poor utilization of nutrients, for example due to changes in the intestinal flora. In the advanced stage, avitaminosis manifests itself with loss of hair, dermatitis and signs of paralysis. The biotin requirement for adults is between 30 – 60 µg biotin per day.
Do you need to examine foods or pharmaceutical products for biotin? In our product portfolio you can find a large range of different test kits and methods for the quantitative detection of vitamin biotin. Which is the right test kit for you?
We offer a wide range of different test kits for biotin examinations: Immune affinity columns, ELISA test kits and microbiological tests as well as dosing standards test kits. The appropriate test can be used according to the laboratory equipment, i.e. whether an HPLC device or a microwell photometer are available.
With the use of immune affinity columns for the pre-purification and concentration of the sample, detection is then carried out with HPLC or LC-MS/MS. Depending on the preparation of the sample there is the option of determining the total biotin content or only the added biotin.
test uses an antibody which is specific for biotin to determine the total biotin content in milk and milk powder; in other matrices the added biotin is determined.